HOMEWORK 7
BIOL/CHEM 5310
due Tuesday, October 29, 2002
Show your work. Use graph paper or a computer for the plots
1. VVP , Chapter 12, page 348, problems 1,3,4,6,8
2. Answer the following questions, using the kinetic data collected below:
|
S (µM)
|
V (µM s-1)
|
V (µM s-1) + Inhibitor
|
|
1
|
6
|
0.654
|
|
2
|
11
|
1.29
|
|
5
|
22
|
3.14
|
|
10
|
33
|
6
|
|
20
|
43.9
|
11.0
|
a) For the uninhibited enzyme, what are Vmax and Km ?
( Show correct units)
b) What are the apparent Vmax and Km, with the inhibitor present?
c) What type of inhibitor is it?
d) If [I] = 2.5 µM, what is Ki ?
3. Design a competitive inhibitor for an ATP hydrolyzing enzyme (ATP --> ADP + Pi). Draw the chemical structure of your inhibitor. (see p 339).
4. In an Eadie-Hofstee plot, v/S is plotted versus v. Plot the following data. Determine Vmax and Km? Use the proper units.
Note: v/S = (-1/Km) V + Vmax/Km
| S (mM) | v (µM/min) |
| 0.08 | 32 |
| 0.1 | 38 |
| 0.16 | 53 |
| 0.2 | 62 |
| 0.4 | 89 |
| 0.8 | 110 |
| 1.6 | 130 |
Last updated
Comments/questions: svik@mail.smu.edu
Copyright 2002, Steven B. Vik, Southern Methodist University