NAD Binding Site

12 residues of alcohol dehydrogenase that are in contact with bound NAD are shown. Look for an ionic interaction between phosphates of NAD and a histidine. There are also hydrophobic van der waals contacts with leucine and valine side chains. Look for hydrogen bonding.

Reference: 1cdo.pdb

Ramaswamy, S., El Ahmad, M., Danielsson, O., Jornvall, H.,Eklund, H.: The Three-Dimensional Structure at 2.1 Angstroms Resolution of a Cod Liver Class I Alcohol Dehydrogenase with Class-Mixed Properties. Medline