HOMEWORK #7
BIOL/CHEM 5312
Due Friday, April 17, 2015
van Holde et al., Chapter 14, p.654-657, problems 14.1, 14.4, 14.5, 14.7, 14.8, 14.9, 14.15, 14.16
14.0 Extra Problem:
Plot the following data in a Scatchard plot, assuming two classes of independent binding sites where n1 = 1 and n2 = 1. Find the affinities for each site.
|
ν |
[A] µM |
|
0.1 |
0.00496 |
|
0.2 |
0.0109 |
|
0.3 |
0.0182 |
|
0.4 |
0.0273 |
|
0.5 |
0.0390 |
|
0.6 |
0.0546 |
|
1.0 |
0.200 |
|
1.5 |
0.826 |
|
1.6 |
1.010 |
|
1.7 |
1.56 |
|
1.8 |
2.47 |
|
1.9 |
5.21 |
Notes:
14.7 You will need to find K24 in terms of K14 and K12. There will be only three data points in the end for each equation. My answers are approximately the same as the book's solution.
14.9 Plot the data and find the steepest slope.