Crystal structure of AcrB multidrug efflux pump

Protomer structure of AcrB, composed of 3 domains.

Transmembrane domain stucture; Each protomer contains 12 transmembrane alpha helices which interact with inner membrane of E Coli.   Pore domain structure; Composed of 4 subdomain structures.   Basic subdomain structure; 2 beta-strand-alpha-helix-beta-strand motifs are directly repeated and sandwiched with each other. TolC docking domain structure; Composed of 2 subdomains containing 4 stranded mixed beta-sheet and 2 antiparallel beta-strands are placed parallel to a hairpin structure from the other half or from the other protomer.

Authors- Satoshi Murakami, Ryosuke Nakashima, Eiki Yamashita, Akihito Yamaguchi.
Title- Crystal structure of bacterial multidrug efflux transporter AcrB.

Nature 419, 587-593 (10 October 2002) (PubMed)

1IWG (PDB)