| Stability and Mutations | Click the buttons to change the display |
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Cartoon
view shows subunit Nqo2 in red colore with the disulfide bond
between the residues Cys144 in the last beta strand and Cys172 in
the loop, both are in spacefilling view and cpk color, this
bonding found to cause the high stability for this subunit.
Trace
view for Subunit 6 which contains the N2 cluster in cpk view with
purple color which is coordinated by Cys residues as showing in
the structure in spacefilling view and red color. Cys45 and Cys46
are too close to each other comparing with others Cys.
This view shows the cluster N2 forms H-bonds with Arg84 and His169,which are both from subunit Nqo4. Mutations of these 2 residues have reported as reason for the unability to detect cluster N2.
Leonid
A. Sazanov, Philip Hinchliffe 2FUG (PDB) |