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The non-canonical PIP box for Pol I is KGLIDYYL. This PIP box is
shown in green, the hydrophobic pocket that binds the PIP boxes
is shown in white. For reference on the back of the protein, the
Lys-164 that can be ubiquitinated is shown in black. Notice the
shape similarity between the PIP box and its binding pocket.
Zoomed
in view of the hydrophobic pocket and the Polymerase I
interaction. Hydrogen bonds have been calculated and show that
there are much fewer than in the other PIP boxes, but there are
several intramolecular H-bonds. Notice that Lys-421 of Pol Ι is
not H-bonded, as expected by the canonical PIP box interactions.
The shape similarity is also similar between the two surfaces. |